Measurement of cholecystokinin octapeptide sulfate (CCK8S) in rat cerebral cortex studied by brain dialysis.
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چکیده
منابع مشابه
Influences of cholecystokinin octapeptide on phosphoinositide turnover in neonatal-rat brain cells.
Cholecystokinin octapeptide (CCK-8) has been shown to be coupled to phosphoinositide turnover in pancreatic acini as well as in a kind of neuroblastoma cell and a human embryonic cell line. Little is known, however, about its link with phosphatidylinositol breakdown in the brain. The brains (minus cerebella) from 1-2-day-old neonatal rats were enzymically dissociated into single cells. The inta...
متن کاملMechanisms of action of peripherally administered cholecystokinin octapeptide on brain stem neurons in the rat.
We have investigated the pathway and the mechanism by which cholecystokinin octapeptide (CCK-8), given systemically, may influence the discharge of brain stem neurons that have an input from the stomach. Extracellular recordings were made from neurons in the nucleus of the solitary tract (NTS), where vagal afferents terminate, and from neighboring regions of the dorsal medial medulla. Gastric d...
متن کاملCholecystokinin and its COOH-terminal octapeptide in the pig brain.
Two components--one resembling intact cholecystokinin in size and charge and immunologic specificity, and the other resembling the COOH-terminal octapeptide of cholecystokinin--have been found in extracts of the pig cerebral cortex. The relative concentrations of the two peptides in the extracts were dependent on the extractant, boiling 0.1 M HCl being more effective than boiling water for the ...
متن کاملEffect of Paraoxon on GABA Uptake by Rat Cerebral Cortex Synaptosomes
Background: It has been suggested that organophosphates may inhibit gamma-aminobutyric acid (GABA) metabolism in synaptosomal preparations. In the present investigation, we have assessed the interaction between paraoxon and the GABA system at synaptic level. Methods: Synaptosomes were prepared from male Wistar rats (200-250 g). Cerebral cortex was dissected and homogenized, then centrifuged at ...
متن کاملIn vivo biosynthesis of cholecystokinin in hog cerebral cortex.
The biosynthesis of cholecystokinin (CCK) in the cerebral cortex of hogs was studied by intracisternal injections of [32S]methionine. At different times (15, 60 and 120 min) after the injection, cortex was isolated and extracted with boiling water and 0.5 M acetic acid. CCK in the extracts was immunosorbed, using an antiserum specific for the COOH-terminal sequence of CCK. Subsequently, the CCK...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1989
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)56096-6